| 质粒名称: | pLVX-XBP1 mNeonGreen NLS |
|---|---|
| 出品公司: | addgene |
| 目录编号: | 115968 |
| 存储实验室: | David Andrews |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-Puro |
| 载体出品公司: | Clontech |
| 空载体大小: | 8102 bp |
| 完整质粒大小: | 9163 bp |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | X-box binding protein 1 |
| 物种来源: | H. sapiens (human), Synthetic |
| 插入大小: | 1061bp |
| 启动子: | CMV |
| 标签/融合蛋白: |
HA tag (N terminal on insert)
mNeonGreen (C terminal on insert)
c-myc NLS (C terminal on insert)
|
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | XbaI (未破坏) |
| 5’测序引物 | AGCTCGTTTAGTGAACCGTCAGATC |
| 3’测序引物 | CAGCGGGGCTGCTAAAGCGCATGC |
|
文献引用方法 |
|
| 材料和方法部分: | pLVX-XBP1 mNeonGreen NLS was a gift from David Andrews (Addgene plasmid # 115968 ; http://n2t.net/addgene:115968 ; RRID:Addgene_115968) |
| 参考文献部分: | Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used for Image Segmentation. Nougarede A, Tesniere C, Ylanko J, Rimokh R, Gillet G, Andrews DW. Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862. 10.1089/adt.2018.862 PubMed 30088945 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
载体简介
载体序列
LOCUS pLVX-XBP1 mNeonGreen NLS 9066 bp ds-DNA circular SYN 12-AUG-2021
DEFINITION ER-stress sensor - XBP1 splicing fluorescent reporter with
mNeonGreen.
ACCESSION .
VERSION .
KEYWORDS pLVX-XBP1 mNeonGreen NLS
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9066)
AUTHORS Nougarede A, Tesniere C, Ylanko J, Rimokh R, Gillet G, Andrews DW
TITLE Improved IRE1 and PERK Pathway Sensors for Multiplex Endoplasmic
Reticulum Stress Assay Reveal Stress Response to Nuclear Dyes Used
for Image Segmentation.
JOURNAL Assay Drug Dev Technol. 2018 Aug 8. doi: 10.1089/adt.2018.862.
PUBMED 30088945
REFERENCE 2 (bases 1 to 9066)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9066
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 158..273
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
enhancer 331..634
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 635..838
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 789..809
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
primer_bind 835..859
/label=LNCX
/note="Human CMV promoter, forward primer"
CDS 973..999
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 1260..1964
/codon_start=1
/product="bright monomeric yellow-green fluorescent protein
derived from LanYFP (Shaner et al., 2013)"
/label=mNeonGreen
/translation="VSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYEE
LNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFEDG
ASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIST
FKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQK
AFTDVMGMDELYK"
regulatory 1512..1521
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 1986..2012
/codon_start=1
/product="nuclear localization signal of human c-Myc
proto-oncogene (Dang and Lee, 1988)"
/label=c-myc NLS
/translation="PAAKRVKLD"
promoter 2032..2531
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
primer_bind complement(2067..2090)
/label=MSCV-rev
/note="Murine stem cell virus, reverse primer"
primer_bind 2464..2483
/label=mPGK-F
/note="Mouse PGK promoter, forward primer"
CDS 2552..3151
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2552..2571)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 3048..3068
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 3165..3753
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(3218..3238)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3636..3647)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3960..4593
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4721..4737)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4721..4737)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4734..4756)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4745..4761
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4769..4799)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4814..4835
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4952..4969)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(5123..5711)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(5203..5222)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5882..6742)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6505..6524
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6743..6847)
/gene="bla"
/label=AmpR promoter
polyA_signal 6895..7029
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6932..6951)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6986..7005
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 7197..7830
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 7877..8002
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8499..8732
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8917..8961
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
ORIGIN
1 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
61 aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga cagagacaga
121 tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag gggggattgg
181 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
241 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
301 tccagtttat cgataagctt gggagttccg cgttacataa cttacggtaa atggcccgcc
361 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
421 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
481 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
541 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
601 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
661 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
721 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
781 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctcg
841 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
901 acaccgactc tactagagga tcgctagcgc taccggactc agatctcgag ctcaagcttc
961 gaattcacca tgtaccccta cgacgtgccc gactacgccg gccttgtagt tgagaaccag
1021 gagttaagac agcgcttggg gatggatgcc ctggttgctg aagaggaggc ggaagccaag
1081 gggaatgaag tgaggccagt ggccgggtct gctgagtccg cagcactcag actacgtgca
1141 cctctgcagc aggtgcaggc ccagttgtca cccctccaga acatctcccc ttggattctg
1201 gcggtattga ctcttcagat tcagagtctg atatcctgtt gggcattcta ataggatccg
1261 tgagcaaggg cgaggaggat aacatggcct ctctcccagc gacacatgag ttacacatct
1321 ttggctccat caacggtgtg gactttgaca tggtgggtca gggcaccggc aatccaaatg
1381 atggttatga ggagttaaac ctgaagtcca ccaagggtga cctccagttc tccccctgga
1441 ttctggtccc tcatatcggg tatggcttcc atcagtacct gccctaccct gacgggatgt
1501 cgcctttcca ggccgccatg gtagatggct ccggatacca agtccatcgc acaatgcagt
1561 ttgaagatgg tgcctccctt actgttaact accgctacac ctacgaggga agccacatca
1621 aaggagaggc ccaggtgaag gggactggtt tccctgctga cggtcctgtg atgaccaact
1681 cgctgaccgc tgcggactgg tgcaggtcga agaagactta ccccaacgac aaaaccatca
1741 tcagtacctt taagtggagt tacaccactg gaaatggcaa gcgctaccgg agcactgcgc
1801 ggaccaccta cacctttgcc aagccaatgg cggctaacta tctgaagaac cagccgatgt
1861 acgtgttccg taagacggag ctcaagcact ccaagaccga gctcaacttc aaggagtggc
1921 aaaaggcctt taccgatgtg atgggcatgg acgagctgta caaggtcgac ggtggatcag
1981 ggggacccgc cgccaagcgc gtgaagctgg actaatctag ataattctac cgggtagggg
2041 aggcgctttt cccaaggcag tctggagcat gcgctttagc agccccgctg ggcacttggc
2101 gctacacaag tggcctctgg cctcgcacac attccacatc caccggtagg cgccaaccgg
2161 ctccgttctt tggtggcccc ttcgcgccac cttctactcc tcccctagtc aggaagttcc
2221 cccccgcccc gcagctcgcg tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta
2281 gtctcgtgca gatggacagc accgctgagc aatggaagcg ggtaggcctt tggggcagcg
2341 gccaatagca gctttgctcc ttcgctttct gggctcagag gctgggaagg ggtgggtccg
2401 ggggcgggct caggggcggg ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc
2461 cggcattctg cacgcttcaa aagcgcacgt ctgccgcgct gttctcctct tcctcatctc
2521 cgggcctttc gacctgcagc ccaagcttac catgaccgag tacaagccca cggtgcgcct
2581 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
2641 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
2701 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
2761 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
2821 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
2881 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
2941 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
3001 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
3061 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
3121 ctggtgcatg acccgcaagc ccggtgcctg accgcgtctg gaacaatcaa cctctggatt
3181 acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg
3241 gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct
3301 cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc
3361 aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca
3421 ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac
3481 tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt
3541 ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt gttgccacct
3601 ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc
3661 cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga
3721 cgagtcggat ctccctttgg gccgcctccc cgcctggaat taattctgca gtcgagacct
3781 agaaaaacat ggagcaatca caagtagcaa tacagcagct accaatgctg attgtgcctg
3841 gctagaagca caagaggagg aggaggtggg ttttccagtc acacctcagg tacctttaag
3901 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa agaggggact
3961 ggaagggcta attcactccc aacgaagaca agatatcctt gatctgtgga tctaccacac
4021 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
4081 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
4141 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
4201 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
4261 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
4321 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
4381 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
4441 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
4501 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
4561 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
4621 agtatttata acttgcaaag aaatgaatat cagagagtga gaggccttga cattgctagc
4681 gtttaccgtc gacctctagc tagagcttgg cgtaatcatg gtcatagctg tttcctgtgt
4741 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
4801 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
4861 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
4921 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
4981 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
5041 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
5101 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
5161 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
5221 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
5281 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
5341 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
5401 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
5461 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
5521 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
5581 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
5641 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
5701 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
5761 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
5821 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
5881 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
5941 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
6001 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
6061 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
6121 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
6181 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
6241 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
6301 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
6361 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
6421 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
6481 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
6541 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
6601 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
6661 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
6721 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
6781 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
6841 ttccgcgcac atttccccga aaagtgccac ctgacgtcga cggatcggga gatcaacttg
6901 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
6961 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
7021 gtctggatca actggataac tcaagctaac caaaatcatc ccaaacttcc caccccatac
7081 cctattacca ctgccaatta cctgtggttt catttactct aaacctgtga ttcctctgaa
7141 ttattttcat tttaaagaaa ttgtatttgt taaatatgta ctacaaactt agtagttgga
7201 agggctaatt cactcccaaa gaagacaaga tatccttgat ctgtggatct accacacaca
7261 aggctacttc cctgattagc agaactacac accagggcca ggggtcagat atccactgac
7321 ctttggatgg tgctacaagc tagtaccagt tgagccagat aaggtagaag aggccaataa
7381 aggagagaac accagcttgt tacaccctgt gagcctgcat gggatggatg acccggagag
7441 agaagtgtta gagtggaggt ttgacagccg cctagcattt catcacgtgg cccgagagct
7501 gcatccggag tacttcaaga actgctgata tcgagcttgc tacaagggac tttccgctgg
7561 ggactttcca gggaggcgtg gcctgggcgg gactggggag tggcgagccc tcagatcctg
7621 catataagca gctgcttttt gcctgtactg ggtctctctg gttagaccag atctgagcct
7681 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
7741 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
7801 ccttttagtc agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa
7861 agggaaacca gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag
7921 aggcgagggg cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga
7981 gagagatggg tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa
8041 aattcggtta aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag
8101 cagggagcta gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag
8161 acaaatactg ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt
8221 atataataca gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa
8281 ggaagcttta gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc
8341 ggccggccgc tgatcttcag acctggagga ggagatatga gggacaattg gagaagtgaa
8401 ttatataaat ataaagtagt aaaaattgaa ccattaggag tagcacccac caaggcaaag
8461 agaagagtgg tgcagagaga aaaaagagca gtgggaatag gagctttgtt ccttgggttc
8521 ttgggagcag caggaagcac tatgggcgca gcgtcaatga cgctgacggt acaggccaga
8581 caattattgt ctggtatagt gcagcagcag aacaatttgc tgagggctat tgaggcgcaa
8641 cagcatctgt tgcaactcac agtctggggc atcaagcagc tccaggcaag aatcctggct
8701 gtggaaagat acctaaagga tcaacagctc ctggggattt ggggttgctc tggaaaactc
8761 atttgcacca ctgctgtgcc ttggaatgct agttggagta ataaatctct ggaacagatt
8821 tggaatcaca cgacctggat ggagtgggac agagaaatta acaattacac aagcttaata
8881 cactccttaa ttgaagaatc gcaaaaccag caagaaaaga atgaacaaga attattggaa
8941 ttagataaat gggcaagttt gtggaattgg tttaacataa caaattggct gtggtatata
9001 aaattattca taatgatagt aggaggcttg gtaggtttaa gaatagtttt tgctgtactt
9061 tctata
//
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